Coding

Part:BBa_K2570002:Design

Designed by: Yuxian Chen   Group: iGEM18_FJNU-China   (2018-09-26)


TyrB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1054
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Site-directed mutation site

TyrB（Pst1：CTGCAG—CTGCAA）

sense primer: GGTGGCTGCAATGCTGAATGACG

Anti-sense primer: TCAGCATTGCAGCCACCACCTG

Source

Phenylalanine aminotransferase Tyrb from Escherichia coli 21B.

https://www.ncbi.nlm.nih.gov/protein/WP_032305522.1?report=genbank&log$=protalign&blast_rank=3&RID=V13P17MW014

References